Clusters-reads correlation?

[cggj]

Hi all,

I am involved in a sequencing project using Illumina/Solexa and I'm analyzing the data. I'm a little confused because the person who did the sequencing says that they got ~16 million clusters for sequencing, when I analyze the data from the seq file I get ~35 million reads. Does this make sense or is there supposed to be a correlation between the numbers?

Thank you!

[kmcarr]

Was the sequencing done paired-end? A paired-end run will produce two reads for each cluster.

[cggj]

Yeap, I know that. The thing is that they made a mistake and they made a single-end run in a paired-end flowcell. I don't know if that is the problem...

[minghui]

may be,I guess

[bioinfosm]

I would guess the first read would sequence out fine for such a case

[drio]

Hi all,

I am involved in a sequencing project using Illumina/Solexa and I'm analyzing the data. I'm a little confused because the person who did the sequencing says that they got ~16 million clusters for sequencing, when I analyze the data from the seq file I get ~35 million reads. Does this make sense or is there supposed to be a correlation between the numbers?

Thank you!

Can you drop here a few lines from the seq file?