Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Hi! New Here...

    Hi All,

    I am a 4th year PhD student in Biostatistics, working on methods for data analysis in RNA-seq data. Right I'm trying to compile comparisons among popular methods to identify the differences and how they work. I realized I need some help with dealing with some of the packages and other methods that are implemented in R. Will have many questions and I am appreciative of all the help I can get! Thanks!

    Andrew

  • #2
    Hi! Is there any chance of including BBMap? I'd love to see how it does in a 3rd-party comparison.

    Comment


    • #3
      I looked over BBMap and it looks to be aligner software (is that correct)? For the current dataset I'm working with this doesn't seem to be applicable (I think it's already aligned). I'm using some RNA-seq data from TCGA which looks like

      Patient Data File

      gene location count

      So the data I compiled for each patient is a 250K x 2 matrix with the patient's raw count for the specific location.

      If I see an opportunity to use BBMap I will definitely look into it! Thanks for making me aware of it!

      Andrew

      Comment


      • #4
        Oh, too bad

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Current Approaches to Protein Sequencing
          by seqadmin


          Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
          04-04-2024, 04:25 PM
        • seqadmin
          Strategies for Sequencing Challenging Samples
          by seqadmin


          Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
          03-22-2024, 06:39 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 04-11-2024, 12:08 PM
        0 responses
        18 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 10:19 PM
        0 responses
        22 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 09:21 AM
        0 responses
        16 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-04-2024, 09:00 AM
        0 responses
        47 views
        0 likes
        Last Post seqadmin  
        Working...
        X