Hello,
we would like to prepare a cDNA Rapid Library from PolyA+ RNA but quality assesment (Agilent Bioanalyzer - attached pictures) showed most likely rRNA contamination. I was told it can interfere following RNA fragmentation so I should try to remove more rRNA from the sample or I can try if the fragmentation is succesful or not. Has anyone any experience with rRNA residue in the sample???
Thank you very much!!!
we would like to prepare a cDNA Rapid Library from PolyA+ RNA but quality assesment (Agilent Bioanalyzer - attached pictures) showed most likely rRNA contamination. I was told it can interfere following RNA fragmentation so I should try to remove more rRNA from the sample or I can try if the fragmentation is succesful or not. Has anyone any experience with rRNA residue in the sample???
Thank you very much!!!
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