Dear all,
I am preparing samples for RNA-SEQ - total RNA was isolated from OCT-Embedded frozen tissue using trizol - I run a RNA integrity check today but there's a lot degradation in some of my samples (See attached, band 1-6). I wonder if these samples could still be used or I have to get some high quality RNA?
BTW, Would too much OCT in the samples affect the isolation process and Quality of RNA?
Thanks.
Cub
I am preparing samples for RNA-SEQ - total RNA was isolated from OCT-Embedded frozen tissue using trizol - I run a RNA integrity check today but there's a lot degradation in some of my samples (See attached, band 1-6). I wonder if these samples could still be used or I have to get some high quality RNA?
BTW, Would too much OCT in the samples affect the isolation process and Quality of RNA?
Thanks.
Cub
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