The SMARTER Pico RNA-seq kit seems to implement a new ribo-depletion technique - ribodepletion of an almost complete sequencing library. (http://www.clontech.com/US/Products/...10020:22372:US).
Has anybody tried this kit or perhaps has an explanation how it could work?
The ribodepletion happens here after a some initial PCR amplification of the library, thus it should be double-stranded at this point. The they add some reagents (ZapR; likely including a bait library) and incubate at 37C one hour and at 72C for 10 minutes. During this treatment the rRNA-sequence containing library fragments are supposedly cut and thus will not be amplified in the following round of PCR which adds the complete Illumina adapters.
Has anybody tried this kit or perhaps has an explanation how it could work?
The ribodepletion happens here after a some initial PCR amplification of the library, thus it should be double-stranded at this point. The they add some reagents (ZapR; likely including a bait library) and incubate at 37C one hour and at 72C for 10 minutes. During this treatment the rRNA-sequence containing library fragments are supposedly cut and thus will not be amplified in the following round of PCR which adds the complete Illumina adapters.
Comment