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Hi all,
I am preparing my RNA samples for SOLiD small RNA sequencing. I analysed my RNA integrity with agilent RNA nano kit and got good results (RIN values >8.00 in all samples). However, when i analyzed the same samples on the small RNA chip, I found out that in 3 out of 10 samples I run, the percentage of miRNAs was 17%, 33%, and 55%, while in the rest of the samples, it was between 1-4%. I have a hard time believing that 55% of my small RNAs are miRNAs, and suspect whether that implies the overabundance of fragments of longer RNAs as a result of degradation. What is your experience in this regard?
cheers,
Bisrat
I am preparing my RNA samples for SOLiD small RNA sequencing. I analysed my RNA integrity with agilent RNA nano kit and got good results (RIN values >8.00 in all samples). However, when i analyzed the same samples on the small RNA chip, I found out that in 3 out of 10 samples I run, the percentage of miRNAs was 17%, 33%, and 55%, while in the rest of the samples, it was between 1-4%. I have a hard time believing that 55% of my small RNAs are miRNAs, and suspect whether that implies the overabundance of fragments of longer RNAs as a result of degradation. What is your experience in this regard?
cheers,
Bisrat