The T4 RNA Ligase in Small RNA Illumina Kit

[mnkyboy]

Does anyone know what the comparable NEB product is for the T4 RNA Ligase is in the Illumina Small RNA kits?

I am assuming it is:

T4 RNA Ligase 2 (dsRNA Ligase) M0239S

Thanks.

[ScottC]

You might want to take a look at the NEBNext RNA sample prep kits. They have a master mix version, and also a version with all the separate enzymes.

Cheers.

[shyong]

I think the T4 RNA Ligase 2 is for 3' adapter ligation. T4 RNA ligase is for 5' adapter ligation.