Hi i have searched the forums but have not found an answer. I have RNA-seq data prepared using dUTP method, paired-end reads and can easily see in IGV when organised by first-in-pair-strand which strand the reads align to. Is there a way to seperate the BAM file so i can generate 2 files, one for each strand for viewing?
The reads have been aligned with STAR.
The reads have been aligned with STAR.