Hello,
i'v assembled with Newmbler a transcriptome of Leukemia.
I have put all my singleton , ncrna sequences, " no Hits" coming out from blast (hg19) in "mirTool" to verify and classify this sequences.
mirTool give me a file with 100% of unclussified and no miRNA, no tRNA , no snRNA found. How is possible this result? I have more than 20.000 sequences, it's so strange. Do you know any software toll to analyze this data that i find difficult to annotate? Particularly i am interested to have a table for the ncRNA (% of tRNA, % of miRNA, ....)
Thank you very much
i'v assembled with Newmbler a transcriptome of Leukemia.
I have put all my singleton , ncrna sequences, " no Hits" coming out from blast (hg19) in "mirTool" to verify and classify this sequences.
mirTool give me a file with 100% of unclussified and no miRNA, no tRNA , no snRNA found. How is possible this result? I have more than 20.000 sequences, it's so strange. Do you know any software toll to analyze this data that i find difficult to annotate? Particularly i am interested to have a table for the ncRNA (% of tRNA, % of miRNA, ....)
Thank you very much
Comment