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  • Hello from NYC

    Hello all. I'm the director of the DNAcore, the sequencing and genotyping facility for researchers at the Mount Sinai School of Medicine in New York City. We have just added a Solexa GA II to our 1 ABI 3730xl and 1 ABI 3130xl sequencer base. It is now installed and we are preparing to run ChIP-Seq samples and sequencing samples from cloned libraries of C. elegan cDNAs. I look forward to learning from and contributing to the forum.

    -David

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  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

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