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Hello!
We are using a SOLiD4 and we would like to sequence
10 multiplexed (marked) patients á 90 fragments á 200-300 bp.
In other words: 1 patient consists of 90 fragments, 200-300 bp each. Each of the 10 patients´samples is marked so that we have a 10x multiplex reaction.
What we would like to do:
After sequencing, separate the patients/fragments of patients and align the runs for the 90 fragments to the corresponding 90 genes, patient by patient.
What software would you recommend?
Thank you in advance.
Best regards
NGS-Jo
We are using a SOLiD4 and we would like to sequence
10 multiplexed (marked) patients á 90 fragments á 200-300 bp.
In other words: 1 patient consists of 90 fragments, 200-300 bp each. Each of the 10 patients´samples is marked so that we have a 10x multiplex reaction.
What we would like to do:
After sequencing, separate the patients/fragments of patients and align the runs for the 90 fragments to the corresponding 90 genes, patient by patient.
What software would you recommend?
Thank you in advance.
Best regards
NGS-Jo