I am trying to get myrna working on our bacterial RNA-seq data.
I first modified a reftools shell script to access EnsemblBacteria for the Streptococcus genome and this seems to work ok.
After running myrna all the directories and files seem to be created but all genes have a p-value of 1.000000e+00 in the pvals.txt file.
The dataset has no replicates so only has one fastq for each of 2 conditions, but I wouldn't expect all p-values to be exactly 1. Do i need more replicates or is something not running correctly within the process?
thanks
adam
I first modified a reftools shell script to access EnsemblBacteria for the Streptococcus genome and this seems to work ok.
After running myrna all the directories and files seem to be created but all genes have a p-value of 1.000000e+00 in the pvals.txt file.
The dataset has no replicates so only has one fastq for each of 2 conditions, but I wouldn't expect all p-values to be exactly 1. Do i need more replicates or is something not running correctly within the process?
thanks
adam
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