Hi all,
I want to mix different index sizes in the same lane in NovaSeq 6000. Is this possible?
Basically I have libraries where I used the Kapa Hyper prep protocol with Truseq dual index adapters and other older libraries where I used Meyer and Kircher protocol also with dual index. The Truseq indexes are 8bp long and the Meyer indexes are 7bp long. I want to pool these libraries made with different protocols and run them together. Is this a problem during sequencing? Can I deal with demultiplexing different index sizes after the run?
Thank you so much for your time
I want to mix different index sizes in the same lane in NovaSeq 6000. Is this possible?
Basically I have libraries where I used the Kapa Hyper prep protocol with Truseq dual index adapters and other older libraries where I used Meyer and Kircher protocol also with dual index. The Truseq indexes are 8bp long and the Meyer indexes are 7bp long. I want to pool these libraries made with different protocols and run them together. Is this a problem during sequencing? Can I deal with demultiplexing different index sizes after the run?
Thank you so much for your time
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