I just tried to determine miRNA features, I computed most but I still don't know how to compute the structure features like the stem length, base paring probability, largest bulge, gap ratio,,etc I tried to get my answer with the Vienna package but I couldn't. What do you suggest. Actually, I tried to extract all the possible features of the secondary structure of miRNA, I used perl and Bioperl but I didn't find any function or package to help me to get the stem length, base paring probability, largest bulge, gap ratio..etc
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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