SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Ion Torrent (http://seqanswers.com/forums/forumdisplay.php?f=40)
-   -   Why Ion Torrent use the Taqman qPCR for sequencing lib quantification (http://seqanswers.com/forums/showthread.php?t=77401)

chaomeizhang 08-02-2017 11:15 AM

Why Ion Torrent use the Taqman qPCR for sequencing lib quantification
 
Hi All,

To be honest, I really do not understand why Ion torrent use the Taqman for library quantification (ION LIBRARY TAQMAN™ QUANTITATION KIT). I asked several people and a FAS, they had no idea. The point is how they design a probe for the assay? I tried to find a manual but failed. Can anybody explain it to me?. Thanks.

All the best.

torben 08-03-2017 03:42 AM

IIRC the probe binds within the P1 adapter, that's why the kit is not compatible with the use of fusion primers with truncated P1.


All times are GMT -8. The time now is 01:32 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.