Hi
I have a RNA-seq (Illumina platform) data without a reference sequence. Then the only option I have is to do a de novo assembly. Followed by gene prediction or mega blast to identified the content of my mRNA.
However if the gene content is unknown. May I know if there is any software available to identified the unknown genes, or any pipeline that I can used.
Say you have hypothetical proteins how am I going to determine that it is a hypothetical protein and what does it functions (any softwares).
Thanks
I have a RNA-seq (Illumina platform) data without a reference sequence. Then the only option I have is to do a de novo assembly. Followed by gene prediction or mega blast to identified the content of my mRNA.
However if the gene content is unknown. May I know if there is any software available to identified the unknown genes, or any pipeline that I can used.
Say you have hypothetical proteins how am I going to determine that it is a hypothetical protein and what does it functions (any softwares).
Thanks
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