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  • Library quality check before capturing

    Dear all

    To check the quality of the genomic DNA library, our collaborators performed a Titration run (1x50) on HiSeq and provided us 50 fasta sequences plus 100'000 fast-q sequences for each library.
    Does this mean, that they loaded the created labrary (prior the exome capturing step) on a flow cell? or how does this work? Does anyone have experience with that? What does titration run mean?

    Is the quality of the library at this stage - prior to the exome capturing - comparable with the quality of the library after the capturing step?

    Thanks a lot for your answers!!
    Last edited by polymerase1986; 04-12-2012, 11:52 AM.

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