Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Question RepeatMasker vs. BLAST - e-Value

    Hi,

    So I was looking at my data with RepeatMasker for some repetitive ~100bp sequences from my customRepLib. I was just looking for their distribution and positions so not exactly masking them and I used the rm-blast search engine that is a modified version of BLAST to work with RM.

    Now I have some (non-repetivie) sequence that maybe assoziated to the repetitive elements and I searched for that one too with RepeatMasker since RM generates a convenient HTML output of its findings and I could just use 1 programm for all the searching.


    The drawback is, RM offers no e-values for its alignments just a Smith-Waterman Score. When I searched for a way to calculate the e-value myself I got doubts if I am using the right tool here and the more I thought abtout it the insecurer I got.


    So the question is twofold:

    1. Is it actually a viable way how I am working or am i missusing RM for my purposes and should stick to the ncbi-blastn+?

    2. Is there a way to get e-values for my RepeatMasker output scores?


    thanks for any input.

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    Yesterday, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 06:57 AM
0 responses
9 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 07:17 AM
0 responses
13 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-02-2024, 08:06 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-30-2024, 12:17 PM
0 responses
23 views
0 likes
Last Post seqadmin  
Working...
X