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-   -   low library yield with Ion AmpliSeq Inherited disease panel (http://seqanswers.com/forums/showthread.php?t=73172)

priyanka_15 12-18-2016 08:08 PM

low library yield with Ion AmpliSeq Inherited disease panel
 
Hi All

I am facing very low yield in library when using 10ng gDNA per pool for Inherited disease panel. I have tried many things like increasing DNA amount, target amplification cycles, ensuring fresh 70% ethanol, drying the beads ( I do 2-3 min. as the place we live is very dry). According to protocol, I should get 300ng/ml-1500ng/ml but I am only getting 132-182ng/ml.

Any ideas or advice?

Thanks


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