Hi
I generated a co-expression map using Kallisto for aligning reads. However, after completing my analysis, when I checked the gene_biotype for the gene list, I only found 3% non-coding RNA. I repeated the analysis with another data from (Skymap), I got similar results.
My question is this normal or Kallisto is not good in aligning non-coding RNA.
I generated a co-expression map using Kallisto for aligning reads. However, after completing my analysis, when I checked the gene_biotype for the gene list, I only found 3% non-coding RNA. I repeated the analysis with another data from (Skymap), I got similar results.
My question is this normal or Kallisto is not good in aligning non-coding RNA.