I am a new for Illumina, recently our lab want to prepare the illumina library by ourselves, without using the current kit. So I have the following questions would like to ask for your help.
1. Is it feasible to directly order the Illumina adapters from the oligos synthesis company? If yes, the adapters should be in which purified level? HPLC?
2. Illumina need to do PCR to amplify the fragmented library. For the primer, which purified level should be set? Also HPLC?
Thanks.
1. Is it feasible to directly order the Illumina adapters from the oligos synthesis company? If yes, the adapters should be in which purified level? HPLC?
2. Illumina need to do PCR to amplify the fragmented library. For the primer, which purified level should be set? Also HPLC?
Thanks.
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