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kainsteven 12-06-2009 10:15 AM

Hello from NuGEN Technologies
 
Steve Kain here. We have recently introduced a solution for RNA-Seq sample prep with inputs of total RNA in the range of 500 pg to 100 ng. The product is double-stranded cDNA in the size range of 50-1,500 bases. I look forward to monitoring this site to learn more about NGS sample prep needs, and progress in the field in general. We are located in the SF Bay Area.

Cheers,
Steve
www.nugeninc.com

tanals 02-01-2010 10:41 PM

Compatibility with SOLiD?
 
Hi Steve,
Is the Ovation RNA-Seq kit compatible with the SOLiD sequencers? We are working with pg quantities of RNA at the moment and will be lucky to get 1ng at the end and want to screen for miRNAs.
Cheers

kainsteven 06-26-2010 07:00 PM

Yes, compatible with SOLiD and 454
 
The product from the Ovation RNA-Seq workflow is ds cDNA, and hence compatible with any library prep system using this starting material. We have several customers that have used the system successfully on SOLiD.

Best,
Steve

acpatel 11-03-2010 09:27 AM

specific question
 
Steve,

Could Prelude be used with OCT-embedded tissue samples (very tiny tissue samples)? For OCT-embedded frozen samples, would one use:
OvationŽ RNA-Seq FFPE System
OvationŽ RNA-Seq
OvationŽ 3'-DGE System

We want have 24 paired samples (12 pairs), and would like to get gene expression data from these samples. Using Qiagen's micro kit, we have RINs and yields ranging from 2.3-5.9 RIN, 9.1 to 2140 mass (ng).

Thanks,
Anand

kainsteven 11-08-2010 07:50 AM

Hi Anand,

Can you please provide some details regarding the impact of OCT treatment on the samples? Ovation RNA-Seq FFPE is preferable if you have degraded samples, and/or cross-linking typically brought about by formalin fixation.

Best,
Steve

josdegraaf 08-09-2011 04:46 AM

Hi,

What is different from the 'normal' rna-seq kit to the FFPE ?

Thanks

josdegraaf 08-09-2011 04:52 AM

I forgot, is there anyone in here that used the FFPE kit with succes?

thanks


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