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cmazzoni 07-21-2011 05:17 AM

Normalization of 16S PCR products
 
Hello everyone,

has anyone ever heard of normalization of 16S PCR products of a meta-sample? I was wondering if it's possible, since the sequences will always be somehow complementary, which could make it difficult to get rid of the similar ones. I would like to have a better representation of the different bacterial groups in my samples, so matter their frequency. Any ideas?

Cheers,

Camila.


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