SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Sample Prep / Library Generation (http://seqanswers.com/forums/forumdisplay.php?f=25)
-   -   Questions sample prep for virus genotyping sequencing (http://seqanswers.com/forums/showthread.php?t=17827)

Juergen F 02-21-2012 09:09 AM

Questions sample prep for virus genotyping sequencing
 
Hi,

Questions:

0 How do I guarantee a certain # of redundant reads per virus target gene area to be utilized later in assembly?

1 - I assume I would need a virus extraction method (Qiagen, Roche) performed upfront prior starting e.g. an Illumina multiplexing workflow with library prep?

2 - Which sample prep methods do match the input bp size requirements best (... output bp size of sample preps)?

3 - What input virus concentration (copies/ IU) do I need to start e.g. the Illumina multiplexing workflow?

Thanks & Greetings
Juergen :)


All times are GMT -8. The time now is 08:18 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.