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simon_seq 02-02-2016 08:42 AM

Automated primer walking
Hi all,
I'm trying to generate a list of oligonucleotide primers for Sanger sequencing, such that for an arbitrarily large DNA sequence I would get an optimised oligonucleotide every ca. 500 bases. How is this best possible? Solutions I have thought along are:
- BatchPrimer 3 (
- Querying NCBI Primer-Blast through R => but is there a tool for that..?


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