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-   -   Can we sequence RNA directly (http://seqanswers.com/forums/showthread.php?t=75257)

SDPA_Pet 04-06-2017 04:03 PM

Can we sequence RNA directly
 
Hello, couple of years ago, I was in a NGS workshop. I was told some company was trying to develop some kits, so we can do metatranscriptomics without building cDNA libraries. I was wondering if this kit is available now?

Or now most sequencing center still use cDNA to do metatranscriptomics.

nucacidhunter 04-06-2017 08:59 PM

I know two technologies for direct transcriptome analysis:
1- nanoString: it can only quantify known transcripts
2- MinIon: the kit currently is offered for early access

cDNA still is the most common method.

Bukowski 04-07-2017 04:00 AM

http://biorxiv.org/content/early/2016/08/12/068809

gringer 04-07-2017 04:17 AM

Quote:

Originally Posted by nucacidhunter (Post 206023)
1- nanoString: it can only quantify known transcripts

nanoString doesn't "sequence" the RNA, unless you're referring to Hyb & Seq (which can sequence novel sequences, but is still in the technology development stages).


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