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-   -   bluepippin size selection for SMRTbells (http://seqanswers.com/forums/showthread.php?t=76218)

seqtechno1 05-26-2017 06:30 AM

bluepippin size selection for SMRTbells
 
Hello,

at which step of the 10kb protocol for preparation of SMRTbell libraries do you run BluePippin 20kb cassette for size selection? I am preparing gDNAs for complete sequencing.

nucacidhunter 05-26-2017 03:44 PM

Current 10kb protocol does not include a Pippin size selection step but if you want to include it then it will be after ligation clean ups. You might follow a Sequel 10 and 20kb hybrid protocol.

weigrc 06-18-2017 11:33 PM

With PacBio 10kb protocol, we got the library sizing around 7kb on agarose gel, no need to do size selection.


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