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-   -   Is a compositional bias in Sequence Content of initial bases in WES reads normal? (http://seqanswers.com/forums/showthread.php?t=95524)

geeneticode 08-07-2020 07:57 AM

Is a compositional bias in Sequence Content of initial bases in WES reads normal?
 
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Per Base Sequence Content

Any experienced Whole Exome Sequencing analyst/experimentalist:

the Per Base Sequence Content of the initial couple of bases from the 3' end of my WES reads have some type of compositional bias. Is this some "harmless" procedural or technical bias occurring during sequencing or is it something to be worried about for the downstream analysis?

https://i.ibb.co/cvj4qS7/per-base-sequence-content.png

HESmith 08-07-2020 11:20 AM

It's entirely normal and does not affect the results.

geeneticode 08-10-2020 10:18 AM

Okay, good to know - thanks for your answer!


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