SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Webinar Series (http://seqanswers.com/forums/forumdisplay.php?f=38)
-   -   Cluster Radius Info in GAII/HiSeq (http://seqanswers.com/forums/showthread.php?t=45671)

som_seq 08-07-2014 04:04 PM

Cluster Radius Info in GAII/HiSeq
 
Hello GAII/HiSeq users,

Can someone tell me what is the typical radius of a cluster in Illumina GAII/HiSeq platform? I believe the total number of bridge amplification cycles (~35) has something to do with the area of the cluster, and assuming clusters are roughly circular in shape, radius (or equivalent) will depend on it. Basically, I am trying to find out, given a cluster density/concentration, how close can any two clusters lie, before there is significant overlap between them resulting in failing filter and poor Q scores? I scoured the net for this info but couldn't get one. Would really appreciate if anyone has any pointers to share.

Cheers and have a great day :) !


All times are GMT -8. The time now is 10:54 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.