SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Literature Watch (http://seqanswers.com/forums/forumdisplay.php?f=10)
-   -   PubMed: Successful Pyrosequencing of GC-rich DNA Sequences by Partial Substitution of (http://seqanswers.com/forums/showthread.php?t=5274)

Newsbot! 05-27-2010 03:00 AM

PubMed: Successful Pyrosequencing of GC-rich DNA Sequences by Partial Substitution of
 
Syndicated from PubMed RSS Feeds

Successful Pyrosequencing of GC-rich DNA Sequences by Partial Substitution of Deoxyguanosine With Deoxyinosine.

Diagn Mol Pathol. 2010 Jun;19(2):123-5

Authors: Bravo LT, Tuohy MJ, Shrestha NK

We experienced significant problems while developing a PyrosequencingTM (Biotage, Uppsala, Sweden) assay to characterize the rifampin resistance-determining region of Mycobacteriumtuberculosis, a target with high guanosine-cytosine content. This paper describes the successful use of a modified pyrosequencing protocol through partial substitution of deoxyguanosine triphosphate with deoxyinosine triphosphate in the polymerase chain reaction.

PMID: 20502190 [PubMed - in process]



More...


All times are GMT -8. The time now is 07:15 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.