SEQanswers (
-   454 Pyrosequencing (
-   -   454 data analysis in Qiime1 (

LMM 01-10-2018 03:03 PM

454 data analysis in Qiime1
I analysing 454 data using Qiime1. I have 4 .fna files that seem to be split into 2 forward read and 2 reverse read files. At what point in the pipeline should I combine them? Before demultiplexing? Combine the OTU tables?

thermophile 01-11-2018 05:59 AM

I don't know qiime well (only used enough to know that I strongly prefer mothur). So can't tell you exactly where to combine but you can't combine OTU tables if you do denovo clustering. In mothur, I combined after demultiplexing, sequence qc, and chimera checking but before aligning and clustering into OTUs

LMM 01-11-2018 06:30 AM

Ok thank you!

All times are GMT -8. The time now is 06:04 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.