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HMorrison 07-02-2012 11:01 AM

96 well PCR clean up
Anyone found a 96 well clean up method that will remove 100 nt/bp and smaller from ~250 bp product? Most give 100 bp as the cutoff, but we've gotten too much small material back with Qiaquick 96. Alternative is Ampure, and it can be tricky to handle in 96 well plate. We'd like to clean up, quantitate, THEN pool and size select to get rid of rest of small material before sequencing. Have tried quantitation, pooling, then clean up and size selection, and dataset yields vary too much. Thanks; Hilary

nexgengirl 07-02-2012 05:55 PM

We've had success with the 96 Well PureLink Pro kit from Invitrogen

Hope this helps.

ECO 07-02-2012 07:32 PM

Nothing will outperform AMPure IMHO. You'll probably have to titrate the cut (specifically the binding time and HOW you mix [static vs constant mixing]) in your own hands, but I would start with 1.3-1.4x for that separation range.

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