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-   -   SOLiD RNA-Seq protocol ver 2 - no PCR products (http://seqanswers.com/forums/showthread.php?t=1438)

wishSEQ 03-29-2009 02:55 AM

SOLiD RNA-Seq protocol ver 2 - no PCR products
 
Hi,
I just finished preparing some libraries for SOLiD RNA-Seq using the new protocol (The one which does not use the Ambion fractionator). After doing a Bioanalyzer run I see that there are no PCR products.

Does anybody have experience with the new protocol? Does it need optimization ? Comments greatly appreciated.


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