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cement_head 04-18-2020 07:31 AM

MiSeq run failed; low clustering, but good QC
Have a strange situation, our latest MiSeq run failed for 16S - from a protocol (EMP @ ArgonneNatLabs) that we use all the time.

The clustering was unusually low (180 vs the expected 800) and we did a KAPA just prior to dilutions (those are correct, I checked). The run QC, Clusters %PF and all the obvious look very good - when the run finished, it did not demultiplex and says "failed".

I've contacted Illumina, but this being a Saturday, I thought I'd see if anyone has seen this before?

cement_head 04-23-2020 08:32 AM

Bad NaOH...

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