SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Illumina/Solexa (http://seqanswers.com/forums/forumdisplay.php?f=6)
-   -   Can you really see miRNAs on gels before PCR amplification (http://seqanswers.com/forums/showthread.php?t=4515)

fisher 03-28-2010 11:57 AM

Can you really see miRNAs on gels before PCR amplification
 
I am new to miRNAseq. We plan to do miRNAseq using small RNA prep V1.0 and I am wondering if we could really see miRNAs on gel after each ligation?

Thanks a lot.

kwaraska 07-21-2010 09:24 AM

miRNA
 
I have never been able to see them. I just do a blind cut and pray a lot!

fisher 07-21-2010 09:44 AM

Thank you!

I finally cut it blindly too. But it worked!



Quote:

Originally Posted by kwaraska (Post 22170)
I have never been able to see them. I just do a blind cut and pray a lot!


link1 07-27-2010 07:37 PM

if you use an adenylated 3' adapter you don't have to perform the small RNA gels and cuts. the adapter specifically will bind to your smalls.


All times are GMT -8. The time now is 07:15 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.