SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Illumina/Solexa (http://seqanswers.com/forums/forumdisplay.php?f=6)
-   -   Homemade illumina oligos and library prep kit (http://seqanswers.com/forums/showthread.php?t=869)

elly 12-01-2008 08:20 PM

Homemade illumina oligos and library prep kit
 
Hi,

I am wondering if we can use the PCR amplification primers which were made at our side.
In theory, this can work properly.

But I am worrying a little bit if there can be some problem with sequencing primer binding and PCR amplification step.

Is there anyone who attempt this?

Thank you in advance.

graveley 01-24-2009 11:39 AM

Yes, as long as the sequences you use are the same as the Illumina sequences, primers you synthesize yourself work fine!

andibody 02-02-2009 09:04 AM

Is this also true for adaptors and sequencing primers?

Thanks
Andi

kkannan 03-24-2009 09:05 AM

For the PE PCR primers, is it necessary to PAGE purify them?


All times are GMT -8. The time now is 03:22 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.