SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Bioinformatics (http://seqanswers.com/forums/forumdisplay.php?f=18)
-   -   cluster density (http://seqanswers.com/forums/showthread.php?t=25682)

andersej 12-12-2012 11:38 AM

cluster density
 
Is it possible that a high cluster density >1300K/mm^2 that may result in difficulties for the MiSeq software to distinguish/misidentify neighboring clusters to intorduce errors i.e. the sequence diverge (maybe due to SNV or indel) and the software cannot identify the correct sequence and this results in a frame shift call?

swbarnes2 12-12-2012 02:28 PM

If 20 reads, from 20 different clusters, going in both directions and all starting from different positions in the genome, and all say there's a frameshift, you have a frameshift. It would be a mistake to conclude anything from a single read, if that's what you are asking.


All times are GMT -8. The time now is 01:33 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.