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-   -   Questions about solexa quality score (http://seqanswers.com/forums/showthread.php?t=329)

baohua100 06-17-2008 05:47 AM

Questions about solexa quality score
 
reads.fq file:

@4:1:518:715
GATACCATAAAAGCTGGATCCTTCTTCAAGCATAA
+4:1:518:715
hhhhhhhhhhhhhhhdhhhhhhhhhhhdRehdhhP

1. How to change character (like 'e' or 'h') to quality score?

2. What's the meaning of this score? How to compute this score ( formula )?

myrna 06-17-2008 08:09 AM

Converting single-character scores to their numeric values
 
Heng Li has provided a nice summary with examples (in Perl) here:

http://maq.sourceforge.net/fastq.shtml


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