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Femta 07-12-2019 04:27 AM

Circulomics Size Selection
 
Hi Community,

I tried today for the first time the Circulomics Size Selection Kit with DNA for a library for MinION.
I started with 5 g DNA with known size (above 40kb, but with fragments lower 10kb)), but after the 30 minutes centrifuging I didn't see a pellet. I processed the sample after that as it was written in the protocol, but the Qubit said, I nearly lost all my sample.

Did I miss something? What can I improve to keep my DNA?
Do you have any other tipps I could follow?

Thank you in advance!


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