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-   -   Total RNA quality for 454 (http://seqanswers.com/forums/showthread.php?t=5232)

nathanjd 05-24-2010 06:19 AM

Total RNA quality for 454
 
2 Attachment(s)
Hi everyone,

First time poster here. I'm looking for some help with a Bioanalyzer trace of total RNA with a view to performing 454 sequencing. I've attached the two traces for my samples and I am worried about a shoulder on the left hand side of the 18s peak and whether this could indicated degredation? These are mosquito samples and I have been told the absence of the 28s peak is expected.

Any help would be greatly appreciated.


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