SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Illumina/Solexa (http://seqanswers.com/forums/forumdisplay.php?f=6)
-   -   Single index adapters for low-plex (http://seqanswers.com/forums/showthread.php?t=85041)

ishmistry 10-09-2018 11:56 PM

Single index adapters for low-plex
 
Hi,

I'm very new to sequencing so apologies if my question is naive.

I am trying to sequence whole human genome samples just to test the reproducibility of my library preparation method. I'm not interested in mutation frequency.

1. Would single-index adapters be good enough for pooling 6-8 samples, or do I need dual index?

2. What depth of coverage would I need to just see if my DNA is good enough to sequence. Could I use MiSeq or would I need HiSeq?

Thanks in advance for any help!


All times are GMT -8. The time now is 04:12 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.