Here's an issue which has arisen with some SureSelect processed libraries for the Illumina: after the hybridization the amount of library is barely within spec -- hence my question about quantitation.
So, the question is which path to take: push the libraries forward as is, with a risk of missing peak sequencer yield due to suboptimal cluster density, or run a few more (how many?) cycles of PCR to increase the concentration but also up the odds of getting PCR duplicates?
So, the question is which path to take: push the libraries forward as is, with a risk of missing peak sequencer yield due to suboptimal cluster density, or run a few more (how many?) cycles of PCR to increase the concentration but also up the odds of getting PCR duplicates?
Comment