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SOLiDance 09-19-2015 06:31 PM

How does SMRT cell detect fluorescent signal?
Hi I have a question about the signal detection in ZMW.
It's said that "The ZMW holes are ~70 nm in diameter and ~100 nm in depth...the wavelength of the light is too large to allow it efficiently pass through the waveguide...". And I believe the excitation fluorescent signal should have even longer wavelength than emitting light. So it should be also impossible to penetrate the ZMW well for the fluorescent signal.

Could anybody share some information/thoughts about Pacbio's detection methods:confused:
Maybe the detectors are located inside each one of the ZMW wells? :eek:Thanks!

Brian Bushnell 09-20-2015 11:06 AM

The reaction occurs at the bottom of the ZMW, presumably against glass or something else transparent. The hole is to narrow for it to go back up into the solution, so it just escapes out the bottom. The video sensors are facing the bottoms of the holes (not embedded within them), according to my interpretation of their promotion videos.

GenoMax 09-21-2015 05:05 AM

@SOLiDance: You could take a look at PacBio's patents on this topic.

SOLiDance 09-21-2015 11:38 PM


Originally Posted by GenoMax (Post 180951)

Thanks a lot!

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