SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Sample Prep / Library Generation (http://seqanswers.com/forums/forumdisplay.php?f=25)
-   -   Urgent: ATAC-seq barcoded primers (http://seqanswers.com/forums/showthread.php?t=92855)

Jieshu 02-06-2020 09:49 AM

Urgent: ATAC-seq barcoded primers
 
Hi guys,

I am new to ATAC-seq, I plan to try this method with Arabidopsis. I am going to order those primers from Supplementary Table 1 (Buenrostro et al., Nat Methods, 2013). For Ad1_noMX and bar-coded primers Ad2.1/2/3..., just order them as what they are? is there any modification for these bar-coded primers? I plan to order them with DESALT from Eurofins, could anyone give me some suggestions?

Thanks,

Jie

luc 02-06-2020 08:22 PM

I believe you can use standard barcoded Nextera primers instead. Preferentially UDI dual indexed versions of the Nextera primers.
Simple desalted oligos will be fine indeed.


All times are GMT -8. The time now is 03:03 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.