SEQanswers (
-   Illumina/Solexa (
-   -   Reagent v3 Miseq stability (

alejandra13 03-06-2020 08:20 PM

Reagent v3 Miseq stability
Hello everyone!

I left reagent V3 in water bath at room temperature for roughly 3 hours. I got a low Q30 score and the cluster density was 1050 K/mm2.

Do you think that the thawing process could affect?

Thanks for your answers!

Saravanan 04-15-2020 12:31 AM

I kept the reagent cartridge in water bath for almost 2 hr and it was OK.
The cluster density 1050 K/mm2 is in normal range.
I think the quality of your sample might not good that's why you got low Q30 score. So, I don't think 3 hrs thawing might affect your seq.

Can you share your bioanalyzer result?

All times are GMT -8. The time now is 01:30 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.