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SofiBaffa 12-28-2015 11:26 AM

Paired end in Ion Torrent
Hi you all!

I need your help... I'm trying to search for a translocation using ion torrent sequencing (i know the genes involved in the translocation, but not the exact point of the break of the chromosomes).
The bioinformatitian that is going to help me with the analysis told me it would be better to perform a paired end sequencing, but I'm not sure if I can do it with ion torrent.
Someone told me that ion torrent performs mate pair, not paired end, but I don't understand de difference.
What's the difference between paired end and mate pair? Can I do it with ion torrent?

Thank you for taking the time to read this and respond :)

Brian Bushnell 12-28-2015 10:16 PM

Ion torrent can do paired-end sequencing. Mate-pair (for clarity, I call it "long-mate pair", or LMP) is a specific type of paired-end sequencing designed for very long insert sizes, and is relevant to assembly, not what you are doing.

SofiBaffa 12-29-2015 05:35 AM

Thank you very much for your response, Brian.
People from life technologies told us that they do not commercialize the kits to perform paired end seq in ion torrent any more. They just have the one for mate-pair seq.
Should I use that kit?

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