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shvetanks 05-31-2017 08:19 PM

Minimum number of samples for RNA-Seq
A reviewer recently asked to define a RNA-Seq study as pilot since we had 32 samples (16 patients each in two comparing groups). Please advise what should be a statistically sound minimum number of samples to be considered for transcriptome studies when comparing two groups?

dpryan 06-01-2017 11:54 PM

The absolute minimum is 3 per group. The "pilot" request is presumably due to the fact that an n of 64 is well within the range of a pilot study for clinical trials. Presuming you're not doing a clinical trial, it'd be appropriate to simply remind the reviewer of that.

shvetanks 06-01-2017 11:59 PM

Thanks a lot dpryan.

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