Hello,
I have 60 amplicons of around 300bp that I need to perform deep sequencing to find mutations.
Can I just synthesize the P7 and P5 adaptors from IDT, ligate onto my amplicons and send out for sequencing? Or do I need to actually buy the Truseq custom amplicon kit (https://support.illumina.com/sequenc...amplicon.html?
Thank you
I have 60 amplicons of around 300bp that I need to perform deep sequencing to find mutations.
Can I just synthesize the P7 and P5 adaptors from IDT, ligate onto my amplicons and send out for sequencing? Or do I need to actually buy the Truseq custom amplicon kit (https://support.illumina.com/sequenc...amplicon.html?
Thank you
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