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-   -   PCR validation of ATAC-seq library (http://seqanswers.com/forums/showthread.php?t=96401)

MzwaneleN 10-09-2020 02:07 PM

PCR validation of ATAC-seq library
 
I have performed PCR validation of an ATAC-seq library targeting known/expected accessible chromatin regions (100bp) but 90% of the products have a smearing banding pattern. Do you perhaps know what could cause this? Could this be inherent to an ATAC-seq library considering that the template is not just one length size but varying lengths?


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