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AndrewThomas 04-27-2013 06:44 AM

Sequencing 400bp 16s Amplicons on the PGM
Dear all,

I would like to know if anyone has tried sequencing 16s rDNA amplicons that are in the correct size range (~320bp) using the Ion Plus Fragment Library Kit and One Touch System 2 then sequenced them using the new 400bp chemistry on a 314 v2 chip. Are there any incompatibilities? How does the data look? Even if they are not 16s amplicons, but other amplicons, I'm interested in your feedback.

Thanks in advance,


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