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-   -   Sequencing 400bp 16s Amplicons on the PGM (http://seqanswers.com/forums/showthread.php?t=29725)

AndrewThomas 04-27-2013 06:44 AM

Sequencing 400bp 16s Amplicons on the PGM
 
Dear all,

I would like to know if anyone has tried sequencing 16s rDNA amplicons that are in the correct size range (~320bp) using the Ion Plus Fragment Library Kit and One Touch System 2 then sequenced them using the new 400bp chemistry on a 314 v2 chip. Are there any incompatibilities? How does the data look? Even if they are not 16s amplicons, but other amplicons, I'm interested in your feedback.


Thanks in advance,



Andrew


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